Average pupil constriction ( n = 6 Pd CO mice), matching the minimal value in time courses shown in f as a function of stimulation frequency ( x axis). Magenta dots and black diamonds represent the EW stimulation effect on the ipsilateral pupil and the contralateral pupil, respectively. Bold dots/diamonds, average across mice ( n = 6). Error bars indicate the s.e.m. across mice. Light dots, average of trials in each mouse. * P < 0.05; multiple-way ANOVA; P (placement) = 1.22 x 10 -20 , P (frequency) = 9.62 x 10 -12 , P (eye laterality) = 1.98 x 10 -4 , P (placement x frequency) = 5.42 x 10 -10 , P (placement x eye laterality) = 8.71 x 10 -3 .
