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Top, spectra of Pd CO activation (solid blue line) and inactivation (dashed blue line), excitation (solid magenta line) and emission (dashed magenta line) spectra of FR-GECO1c 35 and stimulation light properties used for activation/excitation (blue/magenta shaded areas). Bottom (upper left), representative epifluorescence pseudocolor image of neuronal culture transduced with rAAVs encoding Pd CO-EGFP and FR-GECO1c. Bottom (upper right), schematic approach of the cell-attached tight-seal patch-clamp configuration. Bottom, constructs used for spectral multiplexing calcium imaging experiments.
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