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Characterization of each optoGPCRs Ga-protein signaling profile using the GsX assay. Photoactivation of the optoGPCR activates a chimeric Gas subunit harboring the C terminus from one other Ga protein. This chimeric Ga protein activates an AC, which generates cAMP from ATP. cAMP activates a cAMP-dependent luciferase (Luc).

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