Line Plot from Scientific Research

Timelines of the AFM quantities piezo movement and cantilever deflection (bottom), and the FLIM quantities average Flipper-TR intensity and average fluorescence lifetime (top) over all pixels at the indentation site (dark green) and on the outer membrane (light green). The shaded areas correspond to one standard deviation of the Flipper-TR intensities and lifetimes. The vertical black dashed lines represent the timepoints of the first contact of the pipette with the membrane, and the appearance of the pipette in the imaging plane, respectively.

DOI