Representative time series of an intentional rupture of an HFF cell membrane during stimulation with 25 nN indentation and 800 mbar aspiration. The position of the FluidFM cantilever and pipette tip are outlined by the white rectangle and circle, respectively. The green channel shows the cell-permeable calcium-sensitive dye Cal520-AM. The red channel shows the membrane-impermeable dye SR101, which is added to the pipette solution (scale bars, 20 m). This imaging was repeated independently for each of the 320 cells for calcium imaging.
