NMR titration analysis of the N-terminal extended WW12 construct with the high-affinity peptide from SF1 (SF1 WWbs ): two chemical shift perturbation plots are shown for the comparison of 1 H- 15 N (top) and 1 H- 13 C HSQC spectra between the free and bound (1:2) states (spectra shown in Supplementary Fig. 18 ). The most perturbed region corresponds to the WW12 binding interface as in the WW12 titrations; but in the N-terminal extension, two regions appear also affected (due to release from WW12 intramolecular binding). Negative gray bars indicate no data.
