Scatter Plot from Scientific Research

Plot comparing interactors of GFP-tagged PRPF40A-Nter-WT vs PRPF40A-Nter-MUT construct. In the PRPF40A-Nter-MUT construct, the proline-rich sequence was substituted by a polyalanine stretch. To remove background, only proteins that are significantly enriched for the GFP-tagged WT- or Mut-PRPF40A constructs compared to GFP alone are considered. Significantly altered protein interactions are shown in blue (log2 FC > log2(1.5), adjusted p-value < 0.05), non-significant binding differences are shown in gray. Adjusted p values (two-sided) were determined by linear modeling using the limma package. Source data are provided as a Source Data file.

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