ITC results (right) for the titration of the two SF3A1 constructs (shown in left) with the PRPF40A WW tandem; averaged values (bars) and individual data points (black dots) are shown in the graphs. All ITC titrations in ( C , D ) are done at least in duplicates. Note that the SF1/SF3A1 constructs were in the syringe and the WW tandem in the cell so N sites <1 represent more than one WW tandem binding to one peptide. Source data are provided as a Source Data file.
