Bar chart indicating the affinity and stoichiometry values between PRPF40A WW12 and GB1-polyproline peptides of different lengths measured by ITC; the individual data points are shown as black dots. Three selected titrations are shown on the right, displaying the fitting line; full ITC analyses in Supplementary Table 3 and Supplementary Fig. 7 . Note that the peptides were in the cell and the WW tandem in the syringe, so N sites >1 represent more than one WW tandem binding to one peptide.