Bar chart (left) showing the average affinity and individual data points (black dots) of different types of 16-mer peptides contained in the C-terminal region of SF1 (up). The four types of peptides are described in the middle and in Supplementary Fig. 7 . The ITC titration and fitting of the peptide showing the highest affinity (SF1 WWbs ) is on the right. All ITC titrations in ( A , B ) were repeated for each peptide at least twice.