PI/Hoechst staining for cell death in WT, Aplp1 –/– , Lag3 –/– , and Aplp1 –/– / Lag3 –/– cortical neuron cultures, treated with -syn PFF. Scale bar, 100 m. Aplp1-Lag3 deletion exhibited significantly less cell death than in Aplp1 –/– , Lag3 –/– , or WT cultures, treated with -syn PFF. ( p -values: PBS WT vs. PFF WT < 0.0001, PFF group: WT vs. Aplp1 − / − < 0.0001, WT vs. Lag3 − / − < 0.0001, WT vs. Aplp1 − / − / Lag3 − / − < 0.0001, Aplp1 − / − vs. Aplp1 − / − / Lag3 − / − <0.0001, Lag3 − / − vs. Aplp1 − / − / Lag3 − / − < 0.0001). For all experiments, n = 3 independent experiments. For graphs, Data are the means ± SEM. One-way ANOVA with Turkey’s correction. * P < 0.05, ** P <andfeel;0.01, *** P < 0.001, n.s. not significant. Source data are provided as a Source Data file.
