Scatter plot shows a comparison of pore conductances between no SNARE chaperones (black), Munc13-1 (~0.3 µM) (green), and Munc18-1 (~1 µM) (orange) triggered pores; n = 4 (for no SNARE chaperones), n = 13 (for Munc13-1), n = 15 (for Munc18-1) single pores were analyzed; p = 0.0006 (No SNARE chaperones/Munc13-1), 0.0004 (No SNARE chaperones/Munc18-1), 0.005 (Munc13-1/Munc18-1).