Left panel, representative confocal image of primary cortical neurons that were triple labeled for synapsin (Alexa 647-conjugated secondary antibody), SNARE proteins (Alexa 555-conjugated secondary antibody: SNAP-25B (top), Syntaxin1a (middle), Syb2 (bottom) and Munc13-1 (Alexa 488-conjugated secondary antibody). The overlay image shows regions where Munc13-1 coincided with individual SNAREs, as indicated. Blue arrows: co-localization; while arrows: no co-localization. Right panel, representative confocal images of PMA treated primary cortical neuron, under indicated conditions.
