The box plots showing Manders correlation co-efficients M1 (Munc18-1 co-localizing with individual SNAREs as indicated) and M2 (individual SNAREs co-localizing with Munc18-1); n = 5 (Munc18-1/Syntaxin1a and Munc18-1/Syb2), n = 5 (Munc18-1/SNAP-25B). p = 0.004 (Munc18-1/SNAP-25B; M1), 0.018 (Munc18-1/SNAP-25B; M2), 0.335 (Munc18-1/Syntaxin1a; M1), 0.387 (Munc18-1/Syntaxin1a; M2), 0.036 (Munc18-1/Syb2; M1), 0.016 (Munc18-1/Syb2; M2). The box plot minima and maxima represent the 25th and 75th percentiles, the lower and upper whiskers indicate the 5th and 95th percentiles, and the center line and square indicate median and mean, respectively; the Student’s t -test (one-tailed) was performed to compare the two means; * p < 0.05, ** p < 0.01, n.s. non-significance. Scale bar in a and c indicates 5 m. Relevant source data are provided as a Source Data file.
