Bar Plot from Scientific Research

Editing efficacy (correct edits) and precision (correct edits divided by all altered (nonwild-type) reads) were determined from amplicon sequencing. PE4 refers to PE2 + MLH1dn to inhibit MMR, Exo-PE4 refers to Exo-PE + MLH1dn. Each strategy was evaluated using both iPE-N and iPE-C. HEK293T contains a heterozygous SNP 9 bp downstream of the insertion site in the HEK3 locus; for VEGFA , an additional substitution 5 bp downstream of the insertion site was included in the pegRNA design (Fig. 4b ). Selected results of Bonferroni MCT averaged over iPE-N/C (after three-way ANOVA with results reported in the text) are indicated by asterisks; NS P > 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Bars, mean ± s.d. ( n = 3 biological replicates, except for HEK3 C-PE2, N-PE4 and C-Exo-PE4 where n = 2).

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