Scatter Plot from Scientific Research

Groups of five mice were vaccinated i.m. with 1 or 10 ug A/Astrakhan/3212/2020 HA mRNA-LNP (H5 mRNA-LNP) or 10 ug Ovalbumin mRNA-LNP (control). Serum samples were collected 28 days after vaccination and serum antibody binding to HA proteins from diverse of H5 viral strains was determined using a bead-based multiplex binding assay. We measured antibody binding to the HA of ( A ) A/Astrakhan/3212/2020, ( B ) A/red fox/England/AVP-M1-21-01/2020, ( C ) A/pheasant/NY/22-009066-001/2022, ( D ) A/Vietnam/1203/2004, ( E ) A/Hubei/1/2010, and ( F ) A/Indonesia/5/2005. Binding is reported as mean fluorescence intensity (MFI) values. All data are shown as geometric means 95% confidence intervals. Data were compared using two-way ANOVA with Tukeys multiple comparisons test. Values were log-transformed before statistical analysis. Data are representative of 2 independent experiments. A 1 ug vs. 10 ug * P = 0.004; ( B ) 1 ug vs. 10 ug * P = 0.0065; ( E ) 1 ug vs. 10 ug * P = 0.0002; all other comparisons * P < 0.0001. Source data are provided as a Source Data file.

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