Editing efficacy (correct edits) and precision were determined for two insertion types, Flag and FRT, for iPE-N (N) and iPE-C (C) with either the PE2 or the Exo-PE editing strategy in HEK293T cells by amplicon sequencing. Precision was calculated as the proportion of correctly edited reads within all altered (nonwild-type) reads. Correct editing outcomes merely lacking the additional substitution as shown in b were registered separately as correct edits (light blue). Selected results of Bonferroni MCT averaged over iPE-N/C after three-way ANOVA (locus, PE strategy, iPE-N/C) for editing efficacy (results reported in the text) are indicated by asterisks; NS P > 0.05, * P < 0.05, **** P < 0.0001. Bars, mean s.d. ( n = 3 biological replicates).
