Dose-response relationships showing extracellular activation of TREK-2 channel activity by Nb67. Whole-cell TREK-2 currents were recorded at +40 mV and perfused with different concentrations of Nb67. Currents were normalised to those recorded prior to the application of nanobody. No activation of TRAAK or TALK-2 K2P channels was observed, but for the related TREK-1 up to 50% inhibition of channel activity was seen at concentrations of Nb67 > 1 M. (Error bars represent mean S.D; n 3).
