Immunodetection of TREK-2 at the cell surface. TREK-2-mCherry fusions were expressed in HEK-293 cells and stained with either Nb58 (or the highly related Nb57) fused to mVenus via the C-terminus indicated in panel A. Overlay of fluorescent signals shows clear binding at the extracellular surface. No overlapping signals are seen in untransfected controls or with other K2Ps (see Supplementary Fig. S3 ). These results were repeatable in n >= 3 independent replicates.
