Bar Plot from Scientific Research

Whole-cell currents recorded (at +40 mV) from oocytes expressing TREK-2 in the presence of a panel of nanobodies generated against TREK-2. Currents in the presence of extracellular nanobody (0.5 M) were normalised to those in its absence. A threshold of 50% activation or inhibition was set in this screen (dotted lines). Tight binders within this panel were identified on the basis of producing a shift in size exclusion chromatography (SEC-shift) and shown in red. Asterisks mark the functionally active nanobodies plus an inactive tight binder chosen for further investigation. (Error bars represent mean S.D; n >= 5)

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