Bar Plot from Scientific Research

Mirror plot of the singly charged non-tryptic synthetic ProteomeTools 19 peptide VEDPVTVEY measured on a timsTOF (top; mzspec:PXD043844:HLAI_p2_97_178_p2-D1_S1-D1_1_6866.mgf:index:2341:VEDPVTVEY/1) and on an Orbitrap (bottom; mzspec:PXD021013:02446d_GD1-TUM_HLA_133_01_01-3xHCD-1h-R4:scan: 32024:VEDPVTVEY/1) instrument. The spectral similarity measured by the normalized spectral contrast angle based on annotated fragments between the two spectra is 0.68. This illustrates how different timsTOF MS/MS spectra can look compared to Orbitrap data. This randomly chosen peptide was measured several times on the Orbitrap, after which the spectrum with the highest similarity to all other Orbitrap spectra for this peptide was selected (the medoid spectrum). In the timsTOF data the displayed MS/MS spectrum was the only measurement of this peptide. SA normalized spectral contrast angle.

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