Microscopy from Scientific Research

Syncytium formation results of the P681H and P681H/N679K spike protein in Vero E6 cells. Vero E6 cells transfected with vector (NC), S-P681H, S-P681H/GALNT3/T7, S-P681H/N679K or, S-P681H/N679K/GALNT3/T7 plasmids were stained with DAPI (blue) and immuno-stained with anti-Flag antibody (green). The wild-type spike (S-Native) was included as a positive control. Syncytia formation was quantified by the number of nuclei per syncytium (FITC + cells containing multiple nuclei). The N values indicate the number of syncytium counted. Data are presented as mean ± SD. n = 4 independent experiments. For statistical comparisons between means in data ( c , d ), two-tailed P values are calculated by unpaired Student’s t test. Unless otherwise labeled, the displayed P values are the significance between the experimental group and the control group (HEK293T or S-Native). NS: not significant.

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