Microscopy from Scientific Research

GalNAc-T3 and 7 overexpression decreased syncytia formation in Vero E6 cells. Here shows representative views of spike protein-mediated syncytium formation in Vero E6 cells transfected with vector (NC), S-Native, S-R685A, S-Native/GALNT1, S-Native/GALNT3, S-Native/GALNT7 or S-Native/GALNT3/T7 plasmids. Cells were stained with DAPI (blue) and immuno-stained with anti-Flag antibody (green). Syncytia formation was quantified by the number of nuclei per syncytium (FITC + cells containing multiple nuclei). The N values indicate the number of syncytium counted. Data are presented as mean SD. n = 5 independent experiments. Scale bar, 50 m. For statistical comparisons between means in data ( b – f ), two-tailed P values are calculated by unpaired Students t test. Unless otherwise labeled, the displayed P values are the significance between the experimental group and the control group (Native, Vehicle or HEK293T). NS: not significant. Source data are provided as a Source Data file.

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