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Analysis of iron content in sm1, sm1-cat mut , commercial standards (equine holo- and apoferritin (Sigma-Aldrich)). The iron content in sm1 (~41 mol) represents iron adsorbed on both active and non-specific sites, including H 6 , while the iron content in sm1-cat mut (~27 mol) represents iron adsorbed on non-specific sites. Accordingly, the difference in iron content between sm1 and sm1-cat mut (~14 mol) is the amount of iron adsorbed solely on the active site. N = 3 independent experiments. Mean ± s.d. Source Data are provided with this paper.

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