Microscopy from Scientific Research

When expressed in yeast, the native sequence of C16 oxidase (CYP716A297) encodes a protein that has both soluble and aggregated forms, whereas the C16 oxidase expressed from the yeast codon-optimized sequence is cytosolic. By fusing the TMD of C28 oxidase to the N terminus of C16, the TMD C28-C16 fusion protein was correctly anchored to the ER membrane. Images were acquired using a Zeiss LSM 710 confocal microscope. At least three independent experiments were performed. Scale bars, 10 m.

DOI