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Schematic of the proposed metabolic potential of Ca . T. diatomicola (white) and its interactions with Haslea (grey and green), indicating the transfer of fixed nitrogen from the N 2 -fixing symbiont in return for diatom-derived C 4 -dicarboxylic acids, such as succinate. Proteins and corresponding gene names are: Complex I (NADHquinone oxidoreductase, nuoBNEF ), Complex II (succinate dehydrogenase, sdhABCD ), Complex III (cytochrome b/c 1, fbcH_1/2 , fbcF ), Complex IV ( cbb 3 -type oxidase, ccoNOP ), Complex V (ATP synthase, atpABDEGF ); fumarate hydratase ( fumC ); aconitate hydratase ( acnB ); 2-oxoglutarate dehydrogenase ( sucAB , lpd ); succinyl-CoA synthetase ( sucCD ); malate dehydrogenase ( mdh ); isocitrate dehydrogenase ( icd ); citrate synthase ( gltA ); nitrogenase ( nifHDK ) and its ancillary proteins ( nifAENBMQSTUVWXZ ) and ferredoxins ( fdxABN ); rnf complex ( rnfBCD ); dicarboxylic acid transporter ( dctPQM ); pyruvate dehydrogenase ( aceEF , lpd ); pyruvate kinase ( pyk ); malic enzyme ( maeB ); and phosphoenolpyruvate carboxykinase ( pckA ). 2-OG, 2-oxoglutarate; PEP, phosphoenolpyruvate.

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