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Maximum likelihood phylogenetic tree of concatenated bacterial marker genes from the order Rhizobiales, showing the placement of Ca . T. diatomicola within the Hyphomicrobiaceae family (see Methods ). The novel genus Ca . Tectiglobus, comprising Ca . T. diatomicola and its closest relative Ca . T. profundi, is highlighted in pink. Families within the Rhizobiales that contain known N 2 -fixing legume symbionts and their exemplary host plants are shown. The order Parvibaculales was used as an outgroup. Black dots indicate more than 95% bootstrap support. Scale bar indicates amino acid substitutions per site. Plant icons were designed by Freepik ( Neptunia oleracea ) or created with BioRender.com.

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