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Activation of TRPA1 vagal neurons and DBH cNST neurons. AAV-DIO-hM3Dq was targeted bilaterally to the nodose ganglion of Trpa1-cre mice or the cNST of Dbh-cre mice. Control Cre-driver mice received AAV-DIO-mCherry. Mice were challenged with a lethal dose of LPS (see Methods ), and the TRPA1 vagal or the DBH cNST neurons were activated by injection of CNO at 6-h intervals beginning 1 h before injection of LPS (four injections over 19 h).

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