IL-2 (left) and IFNγ (right) in the blood of mice treated with PIP-28ζ CAR T cells with or without B6H12 on day 4, as determined using LEGENDPlex quantitative flow cytometry. Data are mean ± s.d. of n = 5 mice. Statistical analysis was performed using log-rank Mantel–Cox tests ( a and d ), unpaired two-tailed Student’s t -tests ( b ), two-way ANOVA ( c and e ) and one-way ANOVA with Tukey’s multiple-comparison test ( f ).