Human IFNγ in the blood of mice with or without 143B-CD19 tumours, treated with CD19-BBζ, PIP-28ζ or PIP-BBζ CAR T cells on day 4, as determined using LEGENDPlex quantitative flow cytometry. Data are mean ± s.d. of n = 5 (PIP-28ζ or PIP-BBζ with 143B-CD19 tumour), n = 4 (CD19-BBζ with 143B-CD19 tumours), n = 3 (mock with 143B-CD19 tumour and CD19-BBζ, PIP-28ζ or PIP-BBζ without tumours) and n = 1 (mock without tumour) mice.
