Workflow from Scientific Research

An overview of strategies of lineage tracing. Once cells are marked ( i.e. barcoded), they can be exposed to a selected drug concentration to drive the selection of resistant clones (yellow cells). Two types of analyses can then be performed. Prospective analyses where resistant clones ( i.e. lineages) are followed over time during treatment to reconstruct driving mechanisms of drug adaptation. Retrospective analyses where resistant lineages are retrospectively mapped on the untreated condition to reconstruct pre-existing mechanisms of drug resistance.

DOI