Flow cytometry-based quantification of CD45 + CD11b + Ly6G − Ly6C − CD206 − Mg, CD45 + CD11b + Ly6G − Ly6C + CD206 − Mo and CD45 + CD11b + Ly6G − Ly6C − CD206 + BAMs in the Nu-NB and PDOX P3, P8, and P13 TME. For PDOXs P3 and P13 invasive zone and distant normal brain areas were also collected ( n ≥ 3 mice/condition, mean ± SEM, one-way ANOVA with Tukey's HSD correction, **** p < 0.0001, * p < 0.05). See gating strategy in Additional file 2 : Fig S4D