TT-qPCR quantification of transcriptional readthrough at four representative protein-coding genes before (without 5-Ph-IAA) or after (with 5-Ph-IAA) RBM22 degradation in mAID-RBM22 cells. The readthrough ratio in untreated cells (without 5-Ph-IAA) was set to 1. Error bars represent the SD. The p values are determined using the two-tailed unpaired t-test (* p ≤ 0.05; ** p ≤ 0.01; ns, not significant).
