Microscopy from Scientific Research

PyrAte7 a (100 nM, green) internalization and co-localization with the mitochondrial marker tetramethylrhodamine (TMRM, red) after 20 minutes (t20) in HEK293 cells. Fluorophores were excited at a wavelength of 407 nm (PyrAte) or 560 nm (TMRM). Co-localization was evaluated with the Pearson's correlation coefficient (ρ), yielding a strong correlation with ρ=0.838.68An overview of Pearson's correlation coefficients for three different concentrations (20 nM light green, 100 nM green, 300 nM dark green) over several time points reaching from 0-30 minutes shows strong or very strong correlation for all tested conditions from 2 minutes onward (n=3). Scale bar is 20 μm.

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