Bar Plot from Scientific Research

Left: Representative microscopy images of PLZF (red), Ki67 (green), and DAPI (blue) immunodetection on adult F1 seminiferous tubules (P160–P180). Scale bars, 50 μm. Red arrows represent PLZF-positive cells, and red/green arrows represent Ki67 + PLZF + /PLZF + cells. Right: Quantification of Ki67 + PLZF + /PLZF + cells per tubule. Data are represented as the mean ± SEM (black bar), and individual points represent biological replicates. For statistical analysis, P values were calculated by t-test ( b , f , g ). For normality or equal variance of samples failed, Mann–Whitney test was calculated ( c – e ). Statistical significance for all analyses was * P < 0.05; ** P < 0.005; *** P < 0.0005.

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