Microscopy from Scientific Research

HaloTag-LC3 (HT-LC3)-expressing U 2 OS cells were transfected with siNC, siATG2A/B or siANKFY1 for 48 h. Then cells were treated with or without 200 nM Torin 1 for 3 h in the presence of 100 nM BafA1 for 2 h and subjected to the HT-LC3 autophagosome completion assay followed by confocal microscopy. Scale bars, 20 m.

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