Gel from Scientific Research

In-gel fluorescence detection of total cell lysates from WT and ANKFY1 KO U 2 OS cells infected with lentivirus expressing Halo-LC3B, pulse-labeled with 100 nM TMR-conjugated Halo ligand for 30 min, and then treated with Torin 1 (200 nM, 3 h), BafA1 (100 nM, 3 h), or both.

DOI