Schematic diagram outlining liposome co-floatation assay. Liposomes containing 2% Rhod-PE, 30% POPE, 63% DOPC and 5% PI3P were incubated with ATG2A and ANKFY1 as indicated. The incubation mixture was purified by floatation on a three-layer histodenz gradient (35%, 25%, and 0%) and the topmost fraction was collected and analyzed by SDS-PAGE.
