Top row, mean heading direction during the test period versus the wind direction for four example control flies (PFL3>TNT inactive ). TNT inactive denotes expression of a mutationally inactive TNT. Each dot represents the fly’s mean heading in the second and third trials of each wind-direction block. We refer to the absolute difference between this value and the wind direction as the wind-direction error (error). For each fly, the mean error across all six wind directions is indicated above each plot. Data shown as mean ± s.d. in heading across the second and third trials of each block. Bottom four rows show example flies for each of the following genotypes: PFL3>TNT, empty split-Gal4>shibire ts , EPG>shibire ts , and empty split-Gal4>shibire ts flies for which the airflow was turned off.