The database contains analysis of every individual membrane-spanning chain as well as all biologically relevant complexes. In the final database, all structures are aligned relative to the membrane, according to the OPM database, or by running the PPM method. The major steps in constructing the database are: (1) extracting the coordinates and other relevant information from available databases, OPM and PDB; (2) constructing membrane-aligned assemblies and identifying the transmembrane regions therein; (3) identifying the symmetries both within individual chains and between chains in every complex; and (4) computing structural and sequence alignments of related entries to provide a network of relationships. The resultant database contains the final coordinate sets, symmetry analysis results, and structural relationship data. Full details can be found in the Methods andSTAR Methods.
