Number of wind directions that each fly correctly oriented along. Each dot represents one fly. Mean ± s.e.m. across flies is indicated. PFL3>TNT flies oriented along fewer correct directions than PFL3>TNT inactive flies ( P = 0.04 for replicate 1 and P = 5.25 × 10 −7 for replicate 2, two-sided Mann–Whitney U -test; combined P value = 3.90 × 10 −7 , Fisher’s method). PFL3 data are from the 57C10-AD ∩ VT0372202-DBD split-Gal4 line (PFL3 line 1).