Growth reduction rates of Rapalog-treated 3D7/PfCyRPA-recodonized-3xHA and 3D7/PfCyRPA-E148A-3xHA, 3D7/PfCyRPA-I149A-3xHA, 3D7/PfCyRPA-S152A-3xHA, and 3D7/PfCyRPA-I155A-3xHA parasite lines. Growth rates of Rapalog-treated parasite lines were normalized to untreated control parasites of the corresponding parasite line. n = 4 biological replicates (except I149A, here n = 3), error bars indicate standard deviation of the mean. Unpaired t test, p = 0,0005, p = 0,0112, p = 0,0048, and p = 0,1256.
