Affinities of PfCyRPA wild type (WT) and alanine scanning mutants for the preferred glycan targets (α2-6-SLN-Ac and α2-6-Biant-Ac) and for mAb C12. Red and yellow indicate mutants in lectin site 1 and site 2, respectively; orange represents the alanine exchange mutant at position E148, which is part of both binding sites. SPR assay results are an average of at least three replicate experiments shown as average standard deviation inTable S3, with additional mutants that had no significant effect on CyRPA glycan recognition. SPR sensorgrams are shown inFigure S8.
