Density of neurons in the myenteric plexus and submucosal plexus of ChAT-Cre and TH-Cre mice normalized to the number of crypts (n = 3 mice; each data point represents the average of 3 representative fields). ChAT-Cre vs. TH-Cre mice were compared using two-way ANOVA with Sidak’s correction for multiple comparisons for the SI and LI independently. Comparison of different regions in the SI of ChAT-Cre or TH-Cre mice was analyzed using one-way ANOVAs with Tukey’s correction for multiple comparisons. See alsoFigures S1S5andVideo S1. Source data can be found athttps://github.com/mazmanianlab/Griffiths_Yoo_et_al/tree/main/ENS%20quantification.