Expression of a secreted form of RFP in the MpT (uro-Gal4>uas-SPVkgRFP) revealed the presence of the protein in the midgut lumen as revealed by live picture (F) and western blot (G) in flies collected 3 h after Ecc15 oral infection. Red arrows indicate the presence of RFP. PM, peritrophic matrix. Scale bar: 50 μm.