Bar Plot from Scientific Research

BEAS-2B cells were pretreated with punicalagin (50 μM) or glycine (5 mM) for 30 min for the indicated conditions before treatment with MBP-118–45in the presence and absence of mtDNA for 1 h. Heparin was used as a negative control. The cells were fixed and analyzed by TEM. Left: quantification of the plasma membrane damage. Sums of membrane damage are normalized to the length of the plasma membrane shown. Each dot represents a single cell. Right: arrows indicate membrane damage. Arrowheads indicate MBP-118–45peptide aggregates. Scale bars, 250 nm. Images are representative of 2 independent biological replicates. All data are represented as mean ± SEM, andpvalues (∗∗p< 0.01,∗∗∗∗p< 0.0001) were calculated using ordinary one-way ANOVA with Tukey’s multiple comparisons (B and C). See alsoFigure S2.

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