BEAS-2B cells were pretreated with punicalagin (50 μM) for 30 min before treatment with MBP-118-45 in the presence or absence of mtDNA. Heparin and MBP-1*18-45 were used as negative controls. SLO was used as a positive control. Transepithelial electrical resistance (TEER) was measured across epithelial monolayers at the indicated time points. TEER values are shown as fold change to untreated cells. Significances are shown against MBP-118-45 alone (black) or, in the case of punicalagin, against the corresponding condition. Data are representative of 3 independent biological replicates. All data are represented as mean ± SEM, and p values (p< 0.05,*p< 0.01,***p< 0.001) were calculated using ordinary one-way ANOVA with Tukey’s multiple comparisons (B) and two-way ANOVA with Dunnett’s multiple comparisons (C).
