E. coli-GFP (G) and BEAS-2B cells (H) were treated with MBP-118–45in combination with bacterial DNA and human gDNA for the indicated times. Heparin and MBP-1∗18–45were used as negative controls. Cell toxicity was assessed by the loss of GFP (G) or the uptake of PI (H) using flow cytometry. Significance is shown against MBP-118–45alone (black). Data are representative of 4 independent biological replicates. The assays were performed in duplicates. All data are represented as mean ± SEM, andpvalues (ns, not significant;∗p< 0.05,∗∗p< 0.01,∗∗∗p< 0.001,∗∗∗∗p< 0.0001) were calculated using two-way ANOVA with Sidak’s multiple comparisons (A–D) or Dunnett’s multiple comparisons (G and H) and ordinary one-way ANOVA with Tukey’s multiple comparisons (E and F).
