Control or VAP-A/VAP-B double-knockdown INS-1 cells were treated with 50 nM OSW-1 (or vehicle) for 20 min, fixed, and immunostained against insulin and endogenous OSBP. After imaging with a point-scanning confocal microscope, Manders coefficients were quantified from three independent experiments. Each dot represents the mean of one experiment (∗p < 0.05,∗∗p < 0.01; two-tailed, unpaired Student’s t test).
