Microscopy from Scientific Research

Confocal microscopy images of mouse islets with insulin immunostaining shown in cyan and ER-granule contact sites detected with PLA and shown in yellow. Scale bar: 50 m. PLA with Sec61/Rab3 is shown in (E) and PLA with VAP-A/Rab3 in (F). Magnified images below show insulin-containing cells (top pair) and non- cells (bottom pair) containing contact sites, and segmentation/detection of contact sites with Cell Profiler. Scale bar: 5 m. Arrows in magnified images identify PLA puncta overlapping with insulin granules. Scale bars: 50 m/5 m (magnified).

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